Journal: Cellular Microbiology

Article Title: Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells

doi: 10.1155/2023/3286330

Figure Lengend Snippet: Figure 2: C. jejuni modulates NOX1 expression in T84 and Caco-2 cells. qRT-PCR showing expression of NOX1 in (a) T84 and (b) Caco-2 cells. (c) RT-PCR showing expression of NOX1 in uninfected T84 and Caco-2 cells. GAPDH was used as an internal control. (d) RT-PCR showing expression of NOX1 in T84 cells infected with C. jejuni for 24 hours and (e) relative mRNA levels as a percentage from RT-PCR data. (f) Western blotting showing NOX1 in T84 cells infected with C. jejuni for 24 hours and (g) relative protein level as a percentage from Western blotting. Asterisks denote a statistically significant difference (∗p < 0:05; ∗∗p < 0:01; ∗∗∗p < 0:001).

Article Snippet: On the day of reverse transfection, 500μl of Caco-2 cells (105 cells/ml) was seeded in 24-well plates and treated for 24 hours with 30 pmol siRNA from either NOX1 siRNA (sc-43939; Santa Cruz Biotechnology Inc., USA) or Ambion® Silencer Negative Control #1 siRNA (Invitrogen) for the negative control.

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Control, Infection, Western Blot

Journal: Cellular Microbiology

Article Title: Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells

doi: 10.1155/2023/3286330

Figure Lengend Snippet: Figure 3: Proposed structure of the NOX1 complex consisting of NOX1, p22phox, GTP-bound Rac1, NOXA1, and NOXO1. p22phox and other subcellular subunits are assembled to activate catalytic subunit NOX1 which results in the generation of O2 −by oxidising NADPH [17]. Created with http://BioRender.com.

Article Snippet: On the day of reverse transfection, 500μl of Caco-2 cells (105 cells/ml) was seeded in 24-well plates and treated for 24 hours with 30 pmol siRNA from either NOX1 siRNA (sc-43939; Santa Cruz Biotechnology Inc., USA) or Ambion® Silencer Negative Control #1 siRNA (Invitrogen) for the negative control.

Techniques: